Diagnosis of mucocutaneous herpetic infections by PCR without DNA extraction.
نویسندگان
چکیده
منابع مشابه
Detection of herpes simplex virus type 1, 2 and varicella zoster virus DNA in recipient corneal buttons.
AIM To study the value of polymerase chain reaction (PCR) analysis, to detect viral DNA in recipient corneal buttons taken at the time of penetrating keratoplasty (PKP) in patients with an initial diagnosis of herpetic stromal keratitis (HSK). Since HSK has a tendency to recur, an accurate diagnosis of previous HSK could be the reason to start antiviral treatment immediately, thereby possibly d...
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We report the case of a 33 year old man with herpetic bronchitis and bilateral pneumonitis. He presented without mucocutaneous lesions, and his cellular and humoral immunity were not compromised. Diagnosis was established on histological and cytological findings and confirmed by serology. Acyclovir treatment led to a favourable outcome.
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Trematodes are important in economic and public health. Ornithobilharzia turkestanicum (O. turkestanicum) is one of the important economic trematodes in domestic animals. Ornithobilharzia infection in intermediate host (Lymnaea gedrosiana ) can be detected by either exposing snails to light to induce cercarial shedding or by squeezing them between glass slides to detect parasites. The current...
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BACKGROUND Herpes simplex virus infections are very common worldwide. The virus can cause infection in various body parts, especially eyes and the nervous system. Therefore, an early diagnosis and highly sensitive method is very helpful. OBJECTIVES The present study sought to investigate the efficiency of Real-time TaqMan probe PCR in the diagnosis of HSV infection in suspected patients. PA...
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AIMS Sensitive diagnosis of Acanthamoeba infections may prevent the clinical condition from becoming worse. In order to improve the diagnosis tool performances, we studied the implication of the DNA extraction procedures on the detection of Acanthamoeba by real-time PCR. METHODS Acanthamoeba cysts mixed with a tag virus were processed according to different DNA preparation procedures: heat, P...
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 93 2 شماره
صفحات -
تاریخ انتشار 1998